Analysis of cellular energetics performed using Seahorse analyzer and assessment of gene expression related to cell metabolism and respiration ( NRF1, HIF1α, PPARGC1A, ERRα, PKM, PDK1, LDHA, NFKB1, NFKB2, RELA, RELB, REL) revealed that small molecule treatments stimulate AFSCs toward a more energetically active phenotype. Also, some alterations in cell surface marker expression was established by flow cytometry with the most explicit changes in the expression of CD105 and CD117. We observed that combinations of these compounds triggered upregulation of genes involved in pluripotency ( KLF4, OCT4, NOTCH1, SOX2, NANOG, LIN28a, CMYC), but expression changes of these proteins were mild with only significant downregulation of Notch1. For this purpose, we used epigenetically active compounds, such as histone deacetylase inhibitors Trichostatin A (TSA) and sodium butyrate (NaBut), as well as multifunctional molecules of natural origin, such as retinoic acid (RA) and vitamin C (vitC). In this study we investigated the effects of short-term treatments of small molecules to improve stem cell properties and differentiation capability. Human amniotic fluid stem cells (AFSC) are an exciting and very promising source of stem cells for therapeutic applications. ![]() ![]() Department of Molecular Cell Biology, Life Sciences Center, Institute of Biochemistry, Vilnius University, Vilnius, Lithuania.Aistė Zentelytė *, Deimantė Žukauskaitė, Ieva Jacerytė, Veronika V.
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